Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5.
Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and detn. of the affinity const. [on SciFinder(R)]
Références
- Titre
- Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5.
- Type de publication
- Article de revue
- Année de publication
- 2012
- Auteurs
- Basit, Hajra, K Sharma Shivaji, Van der Heyden Angeline, Gondran Chantal, Breyton Cecile, Dumy Pascal, Winnik Francoise M., and Labbe Pierre
- Revue
- Chem. Commun. (Cambridge, United Kingdom)
- Volume
- 48
- Pagination
- 6037–6039
- ISSN
- 1359-7345
Soumis le 12 avril 2018