Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5.

Biotinylated amphipol was used to entrap FhuA (an E. coli outer membrane protein) and immobilize the FhuA-amphipol complex on streptavidin surfaces. Using this assembly, we have successfully devised surface-based assays for studying the recognition of FhuA by pb5 (a bacteriophage T5 protein) and detn. of the affinity const. [on SciFinder(R)]

Références

Titre
Amphipol mediated surface immobilization of FhuA: a platform for label-free detection of the bacteriophage protein pb5.
Type de publication
Article de revue
Année de publication
2012
Revue
Chem. Commun. (Cambridge, United Kingdom)
Volume
48
Pagination
6037–6039
ISSN
1359-7345
Soumis le 12 avril 2018