Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer.

A polymeric film consisting of two distinct monomers such as pyrrole-benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochem. grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aq. electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV-antibody dilns. ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection. [on SciFinder(R)]

Références

Titre
Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer.
Type de publication
Article de revue
Année de publication
2007
Revue
Enzyme Microb. Technol.
Volume
40
Pagination
403–408
ISSN
0141-0229
Soumis le 12 avril 2018