Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer.
A polymeric film consisting of two distinct monomers such as pyrrole-benzophenone and tris(bipyridine pyrrole) ruthenium (II) units was electrogenerated and used for the photochem. grafting of T7 bacteriophages displaying a specific West Nile virus (WNV) epitope. The resulting electrodes were applied to the amperometric detection of WNV antibody at 0 V in aq. electrolyte via secondary peroxidase-labeled antibody, hydrogen peroxide and hydroquinone as the enzyme substrate. A calibration curve based on different WNV-antibody dilns. ranging from 10 to 106 was created with a relatively short response time of 30 s after substrate injection. [on SciFinder(R)]
Références
- Titre
- Amperometric immunosensor for the detection of anti-West Nile virus IgG using a photoactive copolymer.
- Type de publication
- Article de revue
- Année de publication
- 2007
- Auteurs
- Ionescu, Rodica E., Cosnier Serge, Herzog Gregoire, Gorgy Karine, Leshem Boaz, Herrmann Sebastien, and Marks Robert S.
- Revue
- Enzyme Microb. Technol.
- Volume
- 40
- Pagination
- 403–408
- ISSN
- 0141-0229
Soumis le 12 avril 2018