Amperometric Immunosensor for the Detection of Anti-West Nile Virus IgG.

An amperometric immunosensor for the detection of West Nile virus (WNV) IgG was developed. This device was based on the immobilization of T7 phages, which were modified by an addnl. peptide sequence taken from the virus and used as antigen. The electropolymn. of a phage-amphiphilic pyrrole ammonium mixt. previously adsorbed on the electrode surface provided an efficient entrapment of phages in a polypyrrole film. After incubation with a secondary peroxidase-labeled antibody, the immunosensors were applied to the quant. amperometric detn. of WNV-antibody at 0 V vs Ag/AgCl via the redn. of the enzymically generated quinone in the presence of hydroquinone and H2O2. The optimum immunosensor configuration detected low WNV-antibody dilns. down to a titer of 1:107 with an excellent regeneration of the immunosensor response by glycine treatment. [on SciFinder(R)]

Références

Titre
Amperometric Immunosensor for the Detection of Anti-West Nile Virus IgG.
Type de publication
Article de revue
Année de publication
2007
Revue
Anal. Chem. (Washington, DC, United States)
Volume
79
Pagination
8662–8668
ISSN
0003-2700
Soumis le 12 avril 2018