Département de chimie moléculaire

An amperometric immunosensor for the detection of West Nile virus (WNV) IgG was developed. This device was based on the immobilization of T7 phages, which were modified by an addnl. peptide sequence taken from the virus and used as antigen. The electropolymn. of a phage-amphiphilic pyrrole ammonium mixt. previously adsorbed on the electrode surface provided an efficient entrapment of phages in a polypyrrole film. After incubation with a secondary peroxidase-labeled antibody, the immunosensors were applied to the quant. amperometric detn. of WNV-antibody at 0 V vs Ag/AgCl via the redn. of the enzymically generated quinone in the presence of hydroquinone and H2O2. The optimum immunosensor configuration detected low WNV-antibody dilns. down to a titer of 1:107 with an excellent regeneration of the immunosensor response by glycine treatment. [on SciFinder(R)]