Access to a stabilized i-motif DNA structure through four successive ligation reactions on a cyclopeptide scaffold

i-Motifs are largely underexplored tetraplex nucleic acid structures which have been suggested to perform essential biological functions and might constitute future therapeutic targets. i-Motifs generally require acidic conditions to fold in vitro{,} a particularity which significantly complicates the use of native i-motif forming sequences for interaction studies with potential ligands and biological components (e.g. proteins). In this context{,} we report herein on the assembly of a peptide–DNA conjugate capable of folding at room temperature into a stable i-motif structure at neutral pH. To achieve the controlled assembly of the i-motif forming conjugate{,} we developed a new synthetic pathway of four successive orthogonal ligation reactions between bifunctional C-rich DNA strands and a tetrafunctional cyclopeptide scaffold.

Références

Titre
Access to a stabilized i-motif DNA structure through four successive ligation reactions on a cyclopeptide scaffold
Type de publication
Article de revue
Année de publication
2020
Revue
Org. Biomol. Chem.
Volume
18
Pagination
6394-6406
Date de publication
07/2020
Soumis le 27 août 2020